AlignmentofS4segmentsofVACCIS4IIS4IIS4VS4CaI.1VKALRAFRVLRPLRLVSGVISVLRCIRLLRLFKITKYWVKILRVLRVLRPLRAINRASAFFRLERVMRLIKLLSRAECa1.2VKALRAFRVLRPLRLVSGVISVLRCVRLLRIFKITRYWVKILRVLRVLRPLRAINRAITFFRLERVMRLIKLLSRGECa1.3VKALRAFRVLRPLRLVSGVISVLRCVRLLRIEKVTRHWVKILRVLRVLRPLRAINRAITFFRLERVMRLIKLLSKGECa1.4VKALRAFRVLRPLRLVSGVISVERCVRLLRIEKVTRHWVKILRVLRVLRPLRAINRAITFFRLERVMRLIKLLSRGECa2.1VKALRAFRVLRPLRLVSGVISVLRALRLLRIFKVTKYWIKSLRVLRVLRPLKTIKRLLSFLRLERAARLIKLLRQGYCa2.2VKALRAFRVLRPLRLVSGVISVLRALRLLRIFKVTKYWIKSLRVLRVLRPLKTIKRLLSFLRLFRAARLIKLLRQGYCa2.3VKALRAFRVLRPLRLVSGVISVLRALRLLRIFKITKYWMSFLKLFRAARLIKLLRQGYIKSLRVLRVLRPLKTIKRLCa3.1FSAVRTVRVLRPLRAINRVLSVLRTFRLMRVLKLVRFLLRVLRLLRTLRPLRVISRAIRIMRVLRIARVLKLLKMAVCa3.2LSAIRTVRVLRPLRAINRVLSVLRTFRLLRVLKLVRELLRVLRLLRTLRPLRVISRAIRIMRVLRIARVLKLLKMATCa3.3LSAIRTVRVLRPLKAINRVLSVLRTFRLLRVLKLVRFMLRVLRLLRTLRPLRVISRAIRIMRVLRIARVLKLLKMATGreyboxesChargedaminoacidsIndividualtransmembranesegments:~20aminoacidsS4segments:fivetosixpositivelychargedarginins
Alignment of S4 segments of VACC Grey boxes Charged amino acids Individual transmembrane segments: ~ 20 amino acids S4 segments: five to six positively charged arginins
βsubunitβsubunit isan intracellularauxiliary subunitFourβsubunitisoforms(B1-β4)havebeenidentifiedEachisoformhasmultiplesplicevariants
subunit subunit is an intracellular auxiliary subunit Four subunit isoforms (1–4) have been identified Each isoform has multiple splice variants
Structure ofthe α-βinteractionsiteAID:thesequencealignment of theH,NαinteractionsiteQQ-E--L-GY--WI---EAIDBID:sequenceCOOHalignment of theβinteractionsite--E---PYDVVPSMRP-LVGPSLKGYEVTDMMQKALFDFBID
Structure of the − interaction site AID: the sequence alignment of the interaction site BID: sequence alignment of the interaction site
The ysubunitAAnintegral membraneprotein222 amino acids withapredicted molecular mass of25.1 kDaCOOHFourputativetransmembraneBhelices with intracellularly-1located N-and C-termini-2-3TwoextracellularpotentialNglycosylationsitesis-4consistentwiththe observedy-5strong glycosylation of these10255075100subunits%amino acid identity
The subunit An integral membrane protein 222 amino acids with a predicted molecular mass of 25.1 kDa Four putative transmembrane helices with intracellularly located N- and C-termini Two extracellular potential Nglycosylation sites is consistent with the observed strong glycosylation of these subunits
Thesitesof interaction of different regulatory proteins on theintracellularsurfaceoftheα1 subunitof Cay2channelsCOUTSIDEINSIDECO2718+HNCaMKIlCBDPKCSNARESGBYnCas/CaM
The sites of interaction of different regulatory proteins on the intracellular surface of the 1 subunit of Ca V2 channels