Pre ss Release:The 1993 Nobel Prize in Chemistry 13 October 1993 The Royal Swedish Academy of Sciences has decided to award the 1993 Nobel Prize in Chemistry for contributions to the development of methods within DNA-based chemistry, with half to Dr Kary B.Mullis,La Jolla,California,U.S.A.,for his invention of the polymerase chain reaction(PCR) method, and half to Professor Michael Smith,University of British Columbia, Vancouver,Canada,for his fundamental contributions to the establishment of oligonucleotide-based,site- directed mutagenesis and its development for protein studies. PDF文件使用"pdfFactory Pro”试用版本创建香fineprint.com,cn
Press Release: The 1993 Nobel Prize in Chemistry 13 October 1993 The Royal Swedish Academy of Sciences has decided to award the 1993 Nobel Prize in Chemistry for contributions to the development of methods within DNA-based chemistry, with half to Dr Kary B. Mullis, La Jolla, California, U.S.A., for his invention of the polymerase chain reaction (PCR) method, and half to Professor Michael Smith, University of British Columbia, Vancouver, Canada, for his fundamental contributions to the establishment of oligonucleotide-based, sitedirected mutagenesis and its development for protein studies. PDF 文件使用 "pdfFactory Pro" 试用版本创建 ÿ香ÿ www.fineprint.com.cn
Introduction Polymerase chain reaction(PCR)has rapidly become one of the most widely used techniques in molecular biology and for good reason:it is a rapid,inexpensive and simple means of producing relatively large numbers of copies of DNA molecules from minute quantities of source DNA material--even when the source DNA is of relatively poor quality.PCR involves preparation of the sample,the master mix and the primers,followed by detection and analysis of the reaction products PDF文件使用"pdfFactory Pro'”试用版本创建mm,fineprint.com,cn
Introduction Polymerase chain reaction (PCR) has rapidly become one of the most widely used techniques in molecular biology and for good reason: it is a rapid, inexpensive and simple means of producing relatively large numbers of copies of DNA molecules from minute quantities of source DNA material--even when the source DNA is of relatively poor quality. PCR involves preparation of the sample, the master mix and the primers, followed by detection and analysis of the reaction products PDF 文件使用 "pdfFactory Pro" 试用版本创建 öwww.fineprint.com.cn
The applications of Mullis'PCR method are already many.It is for example possible using simple equipment to multiply a given DNA segment from a complicated genetic material millions of times in a few hours,which is of very great significance for biochemical and genetic research.The method offers new possibilities particularly in medical diagnostics,and is used, for example,for discovering HIV virus or faulty genes in hereditary diseases.Researchers can also produce DNA from animals that became extinct millions of years ago by using the PCR method on fossil material. PDF文件使用"pdfFactory Pro”试用版本创建mw,fineprint..com,cn
The applications of Mullis' PCR method are already many. It is for example possible using simple equipment to multiply a given DNA segment from a complicated genetic material millions of times in a few hours, which is of very great significance for biochemical and genetic research. The method offers new possibilities particularly in medical diagnostics, and is used, for example, for discovering HIV virus or faulty genes in hereditary diseases. Researchers can also produce DNA from animals that became extinct millions of years ago by using the PCR method on fossil material. PDF 文件使用 "pdfFactory Pro" 试用版本创建 ÿwww.fineprint.com.cn
原理简介：PCR技术的基本原理是 DNA的半保留复制。由于DNA复制是 半保留的，两条链都可以作为模板。在 体内，DNA复制是周期性的，所以基因 扩增的数量有限；PCR技术在体外利用 人工合成的引物，再加上DNA聚合酶和 些合适的底物和因子，通过对温度的 控制，使DNA不断位于变性、复性和合 成的循环中，达到扩增DNA的目的。 PDF文件使用"pdfFactory Pro”试用版本创建w,fineprint.com,cn
原理简介： PCR 技术的基本原理是 DNA的半保留复制。由于DNA复制是 半保留的，两条链都可以作为模板。在 体内，DNA复制是周期性的，所以基因 扩增的数量有限；PCR技术在体外利用 人工合成的引物，再加上DNA聚合酶和 一些合适的底物和因子，通过对温度的 控制，使DNA不断位于变性、复性和合 成的循环中，达到扩增DNA的目的。 PDF 文件使用 "pdfFactory Pro" 试用版本创建 ÿwww.fineprint.com.cn
何为Pcr技术？ Pcr:Polymerase chain reaction 就是反复进行包括热变性—退火—引物延伸三步骤的循环过 程。 1.热变性：基因组DNA在95度下加热5分，双螺旋结构被热变性 解链为两股单链。 2.退火：将反应混合物降温至55摄氏度，引物与上述单链DNA上 互补的序列杂交在一起，即退火，形成模板—引物复合物。 3.引物延伸：迅速加入TagDNA聚合酶，混匀。置反应混合物于 7O摄氏度，一分钟，在DNA聚合酶作用下，以dNTP为原料，从 引物3端开始，沿着5”一3的方向，按照模板链的序列，合成一 条新DNA链，其序列与模板序列互补。 经上述变性--退火-引物延伸这一循环，双链DNA拷贝数增加一 倍。进行n次循环，拷贝数将增加2的n次方倍，如进行25一30个 循环，拷贝数即扩增上百万倍。 PDF文件使用"pdfFactory Pro" 试用版本创建ww.fineprint.com.cn
何为Pcr技术？ Pcr:Polymerase chain reaction 就是反复进行包括热变性——退火——引物延伸三步骤的循环过 程。 1.热变性：基因组DNA在95度下加热5分，双螺旋结构被热变性 解链为两股单链。 2.退火：将反应混合物降温至55摄氏度，引物与上述单链DNA上 互补的序列杂交在一起，即退火，形成模板——引物复合物。 3.引物延伸：迅速加入TaqDNA 聚合酶，混匀。置反应混合物于 70摄氏度，一分钟，在DNA聚合酶作用下，以dNTP为原料，从 引物3’端开始，沿着5’—3’的方向，按照模板链的序列，合成一 条新DNA链，其序列与模板序列互补。 经上述变性---退火---引物延伸这一循环，双链DNA拷贝数增加一 倍。进行n次循环，拷贝数将增加2的n次方倍，如进行25—30个 循环，拷贝数即扩增上百万倍。 PDF 文件使用 "pdfFactory Pro" 试用版本创建 ÿwww.fineprint.com.cn