Lecture 10 10.5 Genetic Recombination 10.6 Genetic Transformation 10.7 Transduction ·108 Plasmids
Lecture 10 • 10.5 Genetic Recombination • 10.6 Genetic Transformation • 10.7 Transduction • 10.8 Plasmids
Genetic Recombination Donor dna Endonuclease nicks DNA Binding of SsB protein SSB protein Donor Recipient DNA Development of cross-strand exchange Resolution at v sites Resolution at v sites Patches Figure 10.9 Splices
Genetic Recombination Figure 10.9
DNA Transfer Donor Recipient Transformation ◎ Free DNA Virus injection chromosome disruption Transduction DNA-containing vIruses Figure 10.11
Figure 10.11 DNA Transfer
DNA Transfer Conjugation: Plasmid transfer Plasmid-containing donor Conjugation: Chromosome transfer Donor cell with integrated plasmid
DNA Transfer
Selectable Phenotype DNA from Trp cells Trp" cells No growth on Trp cells Recombinants agar lacKing form colonies Figure 10.10 tryptophan on agar lacking tryptophan
Selectable Phenotype Figure 10.10