Chapter 8 Liquid Chromatograph 8.1 Introduction High pressures High speed High performance High sensitivity
Chapter 8 Liquid Chromatograph 8.1 Introduction High pressures High speed High performance High sensitivity
8.2 Principles 1. Classification (lIquid- Solid chromatography Adsorption process Xm+nSa一Xa+nSm XIS
8.2 Principles Adsorption process Xm + n Sa Xa + n Sm (1)Liquid – Solid chromatography n m a n a m X S X S K = 1.Classification
(2)Liquid- Liquid Chromatography Partition Process Xm XS K 、n S
(2) Liquid – Liquid Chromatography Partition Process Xm Xs K k m s s m v v C C = =
(3)lon Exchange Chromatography X++ Y(resins)+- Y*++ X(resins) R/- resin xm [Y-resin
(3) Ion Exchange Chromatography X+ + Y(resins) Y+ + X(resins) X Y resin X resin Y m m − − + + K =
(4)lon pair Chromatography ++YX+Y org XY K X
(4) Ion pair Chromatography a a K - O - X Y X Y + + = X+ a + Ya X+ Yorg