First Repeat Motif Allele APpsystems STR Kits PCR Product Promega STR Kits FCR Product Number of Reference ISFG format ge Sizes(Dye Label) Sizes(Dye Label) Alleles Seen CSLl (40) TAGA 516CO.276-320bdOE5-320bp(6FAM 1.1:287-331 bp (TMR)16317-361 bp (OE) 122512o:196348hp(5FASE196-348 bp (NED)DD:19-348(PED 2.1n6ES:308464bp(IMR) THO 42) TCAT 3-14CO.16204bdOE160-204bp(NEDD:160204bpC 416CO:209257 bp (OE257*(NED 1.1:26-264bp(MR)2.M16:25430bp(MR) 15 (45) ITCTGIITCTAl 1025 PYO/SGM:152-212hym:152-212bp(NED)SE:152-212bp(6FAM1.6ES:123-183bp(IMR) D3s1358 [TCTGIITCTAI 8-21PoOO:97-149b(50:97-149bp(CSE:9-149bp(FAM 2.l6ES:99-151bp(FL 7-18o:134178bp(NED4178bp(PED 11:119163bp(F16:119163 bp OOE) (47) GAT 5-16PoCO253-297b0:253-297bp(6FAM) 1.1:211-255bp(FL16:211-255bpOE) D8s1179 48) [TCTAIrTCT 7-20Po123-175bp0OE-175bp(6FAM)SE:123-175b(vC 2.6ES:203-255bp(TMR DI3S317 93-237bp(NED3237bp*(C 11:157-201bp(FL16:157-201 bp JOE) 16S539 GATA 516COsM233-277b)m:23-277bp*(VC)SE23-27bp(6FAM)1.1:264-308p(FL)16:264308(J0OE AGAA 7-392SGM:26439(264394(NHD)SE:264394(PET 1/6/ES: 286-416bp(FL) D2IS11 (52) Complex I'TCTAI'TCTGI 1241.2 Pro/SGM: 138-256 bpD: 138-256bp(6FAM)SE: 138-256bp(PET) 2.1/1 152820M289314(:28949+VC2941p(AM)A 155-273bp(HFL) D2S1338 (53) [TGCCIITTCCI D19s433 9172 SGMID/SE:106-140) 051918169 enta D (26) AAAGA 2.2-17N/A 16:37649bp(OE Penta E (26) AAAGA 5-24NA 216379474bp(FL) SE3(ACTBP2) (55) Complex AAAG 4.2-37SE:203-33bp(lC) ES: 203-333 bp (oE) Note that the more complex loci have interral variation, which can only be fully charactrith DNA sequence analysis. PCR product sizes are listed for the corresponding allele ranges and STR kits(see Table I for kit abbreviations) based on actual sequence length without any nontempLate addition, nay differ from observed size relative to an internal size standard. Five loci in the Identifiler STR kit are marked with an asterisk ()since mobility modifiers have been added in order to adjust their apparentring electrophoresis TR, short tandem repeat
Gen Bank Accession Physical Position (uly 2003: Physical Position(May 2004 Locus(UniSTS Chromosomal Location NCBI Build 34) NCBI Build 35) TPOX(240638) M6861(11) 2p25.3 thyroid peroxidase, Chr 2 1436 Mb Chr 2 1472Mb lOth intron D2S133800509 AC010136(20) Chr2219.082Mb Chr2218.705Mb D3S1358(148226) AC09539(16) 3p21.3l Chr 3 45.543Mb Chr 3 45-557 Mb FGA(240635) M64982(21) 4q313 fibrinogen, 3rd intron Chr4156.086Mb Chr4155.866Mb D5S818(54700) AC008512(11) 5q232 Chr5123.187Mb Chr5123.139Mb CSFIPO (156169 X14720(12 5q33. 1 c-fms proto-oncogene, Chr 5149, 484 Mb Chr 5 436 Mb 6th intron SE33(ACTBP 00481(26.2) 6q14 B-actin related pseudogene Chr 6 88.982Mb Chr 6 89.043 Mb (none reported) AC004848(13) Chr 7. 401 Mb Chr 7 83.433 Mb D8Sl179(83408 AF216671(13) Chr8125.863Mb Chr8 125.976 Mb TH140639) D00269) hydroxylase. Chr 11 2. 156 Mb Chr11 2. 149Mb Ist intron wA(240640) M2588(18) 12p13.3I von Willebrand Factor, Chr 12 19, Mb Chr 12 5.963 Mb 40th intron Dl3S3170734) AL353628(11) 13g31.1 Chr 13 80, Mb Chr 13 81.620 Mb Penta E(none reported) AC027004(5 15g262 Chr1595.104Mb Chr 15 95.175Mb Dl6S539(4590) AC024591(11) hr 16 86. 1 68 Mb Chr 16 84. Mb Dl8S51(44109) AP1534(18) 18g21.3 Chr 1859. 098 Mb Chr 1859. 100 Mb D1943303588 AC008507(16) 1912 Chr1935.109Mb Chr1935.109Mb D21S11(240642) APO433(29) 21q2l.1 Chr 21 19 476 Mb Chr 21 19476Mb Penta D(none reported) AP001752(13) 2lq223 Chr 21 43.912 Mb Chr 21 43.880Mb Results with two different b new information becomes avai nlm. nih. gow/entrez/query. fcgi? db t nan genome are shown in order to illustrate that the physical position within the reference genome may shift slightly as isacomprehensivedatabaseofsequencetaggedsites(stss)aVAilaBleontheNcbiWebsitehttp:/www.ncbi STR, short tandem repeat
AmpFSTR SGM Plus TM AmpFSTR SEfiler 200bp 0O bp 01b2甲3 200bp 400bp D3s1358 VWA D16s539 D2s1338 D3S s1358 wAD16539D23138 AD8s1179 D21s11 D18s51 D8s1179 SE33 D19433H01 FGA D19s433 THO1 GS500-internal lane standard GS500-internal lane standard AmpFSTR Identifiler TM 200bp 300bp 400bp D8s1179 D21s11 D7S820 CSF1PO D3s1358THo1D13s317D16s539D2s1338 D19s433 VWA TPOX D18s51 D5s818 FGA GS500-internal lane standard
32 Cycles, 50 pg D20s1082D6s474 DI2ATA63 D2251045 D0s1248 Ds1677 DI1$4163 14 DAs2364D9s122 D2s176 D0s1435 D353053D552500 Ds1627D3s4529 D25441 D75974 D6s1017 DA4s2408Ds2157 回四 DI751301 DIGATAl13 D8s853 D20s482 D4s1434 1214
32 Cycles, 50 pg
30 Cycles, 100 pg D20s1082D65474 DI2ATA63 D2251045 D0s1248 DIS1677 DI1$4463 DAs2364D9s122 D0s1435 1D3s3053Dss2500 Ds1627Ds4529 D2s441 Di75974 D6s107 D452408D952157 A DI7S1301 DI GATAll3 D18853 D20s482 D4s1434 1214
30 Cycles, 100 pg