Agar gel film bromocresol purple Mixed faba bean +P Mixed faba bean-P Mixed maize +P Mixed maize-P Faba bean acidified its rhizosphere via proton exudation from roots,whereas maize did not. 2025-6-17 Li et al.,200PWAS104(27):11192-11196
2025-6-17 Prof. Dr. Li L 1 Mixed faba bean +P Mixed faba bean –P Faba bean acidified its rhizosphere via proton exudation from roots, whereas maize did not. Mixed maize +P Mixed maize -P Agar gel film + bromocresol purple Li et al., 2007 PNAS 104 (27): 11192-11196
The malate and citrate concentrations in rhizosphere soil of faba bean were much greater than those of soybean and maize 0.8 ▣Maize ☐Soybean 0.6 ☐Faba bean (I!os.13.ou) 0.4 0.2 0.0 Malate Citrate Malate+Citrate T-aconitic 2025-6-17 Li et al.,Q0RNAS104(27):11192-11196
2025-6-17 Prof. Dr. Li L 2 b b b a a b b a a b b b 0.0 0.2 0.4 0.6 0.8 Malate Citrate Malate+Citrate T-aconitic C o n c e n t r a t i o n i n r h i z o s p h e r e s o i l ( μ m o l • g -1• s o i l ) Maize Soybean Faba bean The malate and citrate concentrations in rhizosphere soil of faba bean were much greater than those of soybean and maize Li et al., 2007 PNAS 104 (27): 11192-11196
Total P uptake by wheat and chickpea grown in a sterilized and non sterile soil under phytate supply Wheaty 12 Chickpea Wheat 731 Chickpea No barrier 8 123 4 Nylon mesh 0 Barrier Nylon No Barrier mesh barrier KH2PO4 Barrier Solid barrier Bte2112a1100 non-sterile 202 ProI.Dr.LIL Li et al.,2003 Plant and Soil 248,297-303
2025-6-17 Prof. Dr. Li L 3 Total P uptake by wheat and chickpea grown in a sterilized and non sterile soil under phytate supply 0 4 8 12 Chickpea Wheat P u p t a k e ( m g / p o t ) 170 139 100 731 123 Barrier Nylon mesh No barrier Barrier KH2PO4 Barrier Sterilization non-sterile Li et al., 2003 Plant and Soil 248, 297-303. No barrier Nylon mesh Solid barrier Chickpea Wheat
30 B ☐Monoculture 25 圆Intercropped (-4 M1-6 lowrl) 20 15 D 10 a 0 Phytate KH2P04 PO Phytate KH2P04 Maize Chickpea Average acid phosphatase activity in rhizoshphere of chickpea supplied with phytate was 2-3 times as much as those of maize. 2025-6-17 Li et al.,2004,ARal of Botany 94:297-303. 4
2025-6-17 Prof. Dr. Li L 4 A cid p h o s p h a t a s e a c tivit y ( μ m ol g-1 F W h-1 ) 0 5 10 15 20 25 30 P0 Phytate KH2PO4 P0 Phytate KH2PO4 Maize Chickpea Monoculture Intercropped A B C D E F a a a a a a b a a a a a Li et al., 2004, Annals of Botany 94: 297-303. Average acid phosphatase activity in rhizoshphere of chickpea supplied with phytate was 2 -3 times as much as those of maize
豆科/禾本科间作提高磷素利用效率的机理 >蚕豆根系释放大量质 子，酸化根际是蚕豆高 效利用土壤中难溶性无 机磷的机理，同时改善 了与之相间作玉米的磷 P Pi p 营养 pH7.5 >鹰嘴豆根系释放大量 的酸性磷酸酶，活化士 arboxylat 壤有机磷，改善小麦的 Fe-P Al- 磷营养； Ca-P in soil >这些机理为磷高效植 s 物和磷低效植物间作套 种，挖掘作物利用磷的 Li et al.,2003 Plant and Soil 248,297-303 生物学潜力提供了新的 器}307No2, 途径 5
2025-6-17 Prof. Dr. Li L 5 H pH7.5 + Pi Pi Pi Pi Pi Pi Ph o s ph at a s e pH 4.0 Li et al., 2003 Plant and Soil 248, 297-303. Li et al., 2004 Annals of Botany 94, 297-303 Li et al., 2007 PNAS 104, 11192-11196 Ø蚕豆根系释放大量质 子，酸化根际是蚕豆高 效利用土壤中难溶性无 机磷的机理，同时改善 了与之相间作玉米的磷 营养； Ø鹰嘴豆根系释放大量 的酸性磷酸酶，活化土 壤有机磷，改善小麦的 磷营养； Ø这些机理为磷高效植 物和磷低效植物间作套 种，挖掘作物利用磷的 生物学潜力提供了新的 途径. Pi Pi Pi H+ H+ H+ Fe-P, Al-P, Ca-P in soil Organic P in soil 豆科/禾本科间作提高磷素利用效率的机理 Carboxylates