mRN cytoplasmic 5 ribosome RNA-binding proteins CYTOSOL CYTOSOL nuclear pore protein exchange -s:w >M 奇 NUCLEUS NUCLEUS mRNA RNP particle poly-A-binding protein (B) 200nm
mRNA的输出
2.1核蛋白运输机 →基本概念 ◆核蛋白( nuclear protein) ◆核定位信号( nuclear localization signals,NLS) ◆核输出信号( nuclear export signals, NES ◆输入蛋白( importin) ◆输出蛋白( exportin
2.1核蛋白运输机制 基本概念 ◆核蛋白(nuclear protein) ◆核定位信号(nuclear localization signals ,NLS) ◆核输出信号(nuclear export signals, NES) ◆输入蛋白(importin) ◆输出蛋白(exportin)
核定位 TABLE 19-6 Amino Acid Sequences That Act as Signals for Translocation to the cell Nucleus Type of Nuclcar Location of Deduced Nuclear Signal Protcin Signal in Protein Localization Sequence Single large t antigen of Residues 126-132 Pro Lys Lys Lys Arg Lys Val SV40 virus Adenovirus Ela C-terminus Lys Arg Pro Arg Pro Influenza virus nucleo- Near C-terminus; residues AlaAla Phe Glu Asp leu Arg Val Leu Ser ein 336-345 Bipartite Xenopus nucleoplasmin Near C-terminus; residues Lys Arg Pro Ala Ala Thr Lys I ys Ala Gly 155-171 GIn Ala Lys I ys LysLys Leu Xenopus NI nuclear Internal; residues 534-550 Lys Arg Lys Thr Glu Glu Gu Ser Pro Lcu protein L ys Asp Lys Asp Ala L ys Lys Yeast Swi s tran Internal; residues 636-652 Lys Lys Tyr Glu Asn ValVal llc Lys Arg scription factor Ser Pro Arg Lys Arg Gly Arg Mouse poly-ADP-ribosc Internal; residues 208-224 Arg Lys Gly Asp Glu Val Asp Gly Thr Asp polymerase Glu Val Ala L ys Lys Lys Ser Chicken estrogen Intcrnal residues 250-266 Arg Lys Asp Arg Arg Gly Gly Glu Met Met L ys Gln Lys Arg GIn Arg glu ' Boldface the basic residues thought ro be essenti.l fe r nuclear uptake BIon(:C. Dingwall and R. Laskey, 1986, Ann. Reu. Cell Biol. 2: 367-390: and C. Dingwall and R. Laskey. 1991,Trend. bem.Sci.16:478-481
核定位信号
Nuclear localiza antigen Nucleoplasmin ○0以
Nuclear localization signals
→核蛋白的输入 ◆核质蛋白 nucleoplasmin)实验 ◆核蛋白输入机理 ●输入蛋白 ●Ran蛋白:小 GTPase RangAP: RangTp激活蛋白细胞质 RCC1. Ran nucleotide exchange factor1细胞核中
核蛋白的输入 ◆核质蛋白(nucleoplasmin)实验 ◆核蛋白输入机理 ●输入蛋白 ●Ran蛋白:小GTPase RanGAP:RanGTP激活蛋白:细胞质 中 RCC1：Ran nucleotideexchange factor1.细胞核中