Aim assess whether a H. pylori infection could affect growth in early life investigate the interplay between H. pylori and normal gut microbiota during early stages of life understand the influences of H pylori and normal gut microbiota on the development of metabolic and immune systems by assessing the metabolic hormones and cytokines
Aim • assess whether a H. pylori infection could affect growth in early life • investigate the interplay between H. pylori and normal gut microbiota during early stages of life • understand the influences of H. pylori and normal gut microbiota on the development of metabolic and immune systems by assessing the metabolic hormones and cytokines
Methods-①分组&取样 SPF SPFH GF GFH py gut microbiota male mice, 4 group, N12/per group, age 2-4 weeks sacrificed in2、8、16 weeks post- colonization fasting plasma(空腹血浆) was used for hormone and cytokine analysis
Methods——①分组＆取样 SPF SPFH GF GFH H. pylori - + - + gut microbiota + + - - • male mice，4 group，N=12/per group，age 2-4 weeks • sacrificed in 2 、8、16weeks post-colonization • fasting plasma （空腹血浆）was used for hormone and cytokine analysis
Methods②检测指标 hormone and cytokine eight gut hormones ghrelin, leptin, amylin, insulin Metabolic system GLP-1,GIP PYY and pp (important regulators of food intake, energy expenditure and body mass) Immune system eotaxin-1
Methods——②检测指标 hormone and cytokine Metabolic system eight gut hormones：ghrelin, leptin, amylin, insulin, GLP-1,GIP, PYY and PP（important regulators of food intake, energy expenditure and body mass） Immune system eotaxin-1
RESULT--GE小鼠体重低于SPF小鼠 Body Weight normal gut microbiota plays an Important role in determining energy harvest from the diet and P. SPF energy storage in its host FH Duration(weeks)
• normal gut microbiota plays an important role in determining energy harvest from the diet and energy storage in its host RESULT——GF小鼠体重低于SPF小鼠
RESULT--GFH小鼠体重低于GF小鼠 Body Weight in the absence of normal gut microbiota, H pylori caused weight loss -Q-0SPF SPFH 正常微生物菌群对幽门螺杆菌 导致的体重下降有减缓作用 16 Duration(weeks)
RESULT——GFH小鼠体重低于GF小鼠 • in the absence of normal gut microbiota, H. pylori caused weight loss. • 正常微生物菌群对幽门螺杆菌 导致的体重下降有减缓作用