A 回 S Repair of Adult Rat Corticospinal Tract by Transplants of olfactory cience Ensheathing Cells Ying li et al NAAAS Science277,2000(1997 DO|:10.1126/ scIence.2775334.2000 Fig3(A) Electron micrograph of astrocyte-like OECS (a).(B)Across section taken from the center of the lesion and myelinated by thick, peripheral-type myelin formed Schwann like Fig2 The mass of transplanted OECs(red) OECS(S).(C)Transitional internode(Ro, elongated along the axis of the host CST. scale in the transplant) is myelinated by a bar, 200 um. Schwann-like OEC (arrowhead) LiY, Field PM, Raisman G. Repair of adult rat corticospinal tract by transplants of olfactory ensheathing cells. Science. 1997 Sep 26: 277(5334): 2000-2
16 Li Y, Field PM, Raisman G. Repair of adult rat corticospinal tract by transplants of olfactory ensheathing cells. Science. 1997 Sep 26; 277 (5334): 2000-2. Fig1 Destruction of the left corticospinal tract (arrow). Scale bar, 0.5 mm Fig2 The mass of transplanted OECs (red) elongated along the axis of the host CST. Scale bar, 200 μm. Fig3 (A) Electron micrograph of astrocyte-like OECs (a) . (B) A cross section taken from the center of the lesion and myelinated by thick, peripheral-type myelin formed Schwann like OECs (s). (C) A “transitional” internode (Ro, in the transplant) is myelinated by a Schwann-like OEC (arrowhead)
a The Journal of Neuroscience Fibroblasts 11120. The Journal of Neuroscience, October 25, 2006. 26(43 ): 11120-11130 Development/Plasticity/Repair Olfactory Ensheathing Cells Do Not Exhibit Unique Migratory or Axonal Growth-Promoting Properties after Spinal Cord injury Paul Lu, 2 Hong Yang,' Maya Culbertson, Lori Graham, 'A Jane Roskams, and Mark H Tuszynski.2 Department of Neuroscience, University of California at San Diego, La Jolla, California 92093, vEterans Affairs Medical Center, San Diego, California 92161, and Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4 fill of lesion cavity small volumes IeGleu acutely antel luly Lu P et al. Olfactory Ensheathing Cells Do Not Exhibit Unique migratory or Axonal Growth- Promoting Properties after Spinal Cord Injury. J Neurosci. 2006: 26: 11120-30 17
17 Lu P et al. Olfactory Ensheathing Cells Do Not Exhibit Unique Migratory or Axonal GrowthPromoting Properties after Spinal Cord Injury. J Neurosci. 2006;26:11120-30. Fig 7. OECs do not form tracts after injection at greater distances from lesion, or after injection of small volumes. Fig 8. Other cell types form tracts to the lesion site when injected acutely after injury Fig 6. Time course of OEC tract formation and fill of lesion cavity
The journal of Neuroscience, October 25, 2006. 26(43): 11120-11130 oWe do not find a propensity of OECs to migrate or otherwise support directed axonal extension or corticospinal tract regeneration fIndings of the present spinal cord injury model yield no evidence that OECs stimulate axon growth more extensively than other cell types o The rationale for using these cells in extensive human experimentation now underway is therefore unclear Lu P et al. Olfactory Ensheathing Cells Do Not Exhibit Unique Migratory or Axonal Growth Promoting Properties after Spinal Cord Injury. J Neurosci. 2006: 26: 11120-30
18 ◆We do not find a propensity of OECs to migrate or otherwise support directed axonal extension or corticospinal tract regeneration. ◆Findings of the present spinal cord injury model yield no evidence that OECs stimulate axon growth more extensively than other cell types. ◆The rationale for using these cells in extensive human experimentation now underway is therefore unclear. Lu P et al. Olfactory Ensheathing Cells Do Not Exhibit Unique Migratory or Axonal GrowthPromoting Properties after Spinal Cord Injury. J Neurosci. 2006;26:11120-30
CELL SOURCES Human induced Human embryonic Fetal pluripotent stem cells(hiPSCs stem cells(hESCs) brain tissue Patient CEL skin cells or broblasts Instruct to glial fate Dissect regionally enriched phenotypes Glial progenitor cells (GPCs CELL SELECTION A2B5+/PSA-NCAM. Magnetic activated cell sort CD140a+(PDGFaRt) Fluorescence activated cell sort CD140a*/CD9+ Isolated glial progenitors CELL TRANSPLANTATION Adult child Glial Progenitor Cell-Based Treatment and Modeling of Neurological Disease Science26 Octo ber2012:338(6106),491-495
19 Glial Progenitor Cell-Based Treatment and Modeling of Neurological Disease. Science 26 October 2012: 338 (6106), 491-495