3作用机制: (ADH与小管上皮细胞V2受体结合→腺苷酸环化酶活性↑ →cAMP生成↑→蛋白激醃激活→丶含水孔蛋自AQP2小泡镶嵌 在管腔膜→水通道形成个→>水通透性↑→重吸收↑ 小管液 血液 ADH缺乏时管腔膜 上水通道集中在其衣 蛋白激酶 ATP 被凹陷处,以后形成 含水通道的 小泡内移 CAMP 圈 吞饮小泡。腔膜面水通 磷酸蛋白 道消失,水不再通透 图8-19抗利尿激素的作用机制示意图
3.作用机制: (ADH)与小管上皮细胞V2受体结合→ 腺苷酸环化酶活性 → cAMP生成 → 蛋白激酶激活→ 含水孔蛋白AQP-2小泡镶嵌 在管腔膜 → 水通道形成 → 水通透性 → 重吸收 。 ADH 缺乏时,管腔膜 上水通道集中在其衣 被凹陷处, 以后形成 吞饮小泡。腔膜面水通 道消失，水不再通透
2003年诺贝尔化学奖获得者彼得·阿格雷 Peter Agre Johns hopkins Universit School of medicine Baltimore, MD, USA 东方网10月9日消息:10月8日,瑞典皇家科学院在瑞典首都斯德哥尔摩宣布,将2003 年诺贝尔化学奖授予美国科学家彼得·阿格雷和罗德里克·麦金农,分别表彰他们发现细 胞膜水通道,以及对离子通道结构和机理研究作出的开创性贡献
Peter Agre Johns Hopkins University School of Medicine Baltimore, MD, USA
Peter Agre, born 1949(54 years)in Northfield, Minnesota (US citizen). Medical Doctor 1974 at Johns Hopkins University School of Medicine, Baltimore. USA Professor of biological Chemistry and professor of medicine at Johns Hopkins University School of Medicine, Baltimore, USA That the body's cells must contain specific channels for transporting water was suspected as early as the middle of the nineteenth century. However, it was not until 1988 that Peter Agre succeeded in isolating a membrane protein that, a year or so later, he realised must be the long-sought-after water channel
Peter Agre, born 1949 (54 years) in Northfield, Minnesota (US citizen). Medical Doctor 1974 at Johns Hopkins University School of Medicine, Baltimore, USA. Professor of Biological Chemistry and Professor of Medicine at Johns Hopkins University School of Medicine, Baltimore, USA. That the body's cells must contain specific channels for transporting water was suspected as early as the middle of the nineteenth century. However, it was not until 1988 that Peter Agre succeeded in isolating a membrane protein that, a year or so later, he realised must be the long-sought-after water channel
o5 min 1.5min 2.5mn 3.5mn Cell with aqua porin Cell without aquaporin Peter Agre's experiment with cells containing or lacking aquaporin. The aquaporin is necessary for making the cell absorb water and swell
Peter Agre’s experiment with cells containing or lacking aquaporin. The aquaporin is necessary for making the cell absorb water and swell
Water permeability of aquaporin-1(AQP1)expressed in Xenopus oocytes. When transferred to hypo-osmolar buffer for 2 min, control water-injected oocytes exhibit negligible swelling(left). Under the same conditions, oocytes previously injected with AQPI CRNA rapidly swell and explode ( right)
Water permeability of aquaporin-1 (AQP1) expressed in Xenopus oocytes. When transferred to hypo-osmolar buffer for 2 min, control water-injected oocytes exhibit negligible swelling (left). Under the same conditions, oocytes previously injected with AQP1 cRNA rapidly swell and explode (right)