Science Niklason et al. Tissue-Engineered Lungs for in vivo NAAAS Implantation Science329,538(2010); ascular Endothelium Pulmonary Epithelium Vascular Edad perfusion E Breathing loop Medium Syringe exchange Trachea B 将供体肺组织进行去细胞化处理,得到去细胞化支架 去细胞化支架与种子细胞生物反应器中共培养 培养数周的组织工程体内移植 三强图学出到
Niklason et al. Tissue-Engineered Lungs for in Vivo Implantation. Science 329,538 (2010); •将供体肺组织进行去细胞化处理，得到去细胞化支架 •去细胞化支架与种子细胞生物反应器中共培养 •培养数周的组织工程体内移植
Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system Biomaterials 33(2012)7756e7764 0 hours 6 hours 24 hours 36 hours a-c:0.5%SDs,0.25%SDs,1% Triton X100/0.1% Ammonium Hydroxide d:不同去细胞化的方法与未去细胞肾 脏的DNA比较 e-h:native, 0.5% SDs,(g)0.25% SDs, and ( h1% Triton x 100/0.1%Ammonium Hydroxide 在组织染色上的比较 去细胞化支架的方法依然存在着很多 改进的方面。 三强图学出到
Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system Biomaterials 33 (2012) 7756e7764 a-c:0.5% SDS，0.25% SDS，1% Triton X- 100/0.1% Ammonium Hydroxide d:不同去细胞化的方法与未去细胞肾 脏的DNA比较 e-h:native，0.5% SDS，(g) 0.25% SDS，and (h) 1% Triton X- 100/0.1% Ammonium Hydroxide 在组织染色上的比较。 去细胞化支架的方法依然存在着很多 改进的方面
Decellularization methods of porcine kidneys for whole organ engineering using a high-throug hput system Biomaterials 33(2012)7756e7764 a:缩的去垢剂和缓冲液。b:去离子水加入。c:蠕动泵d:三通管 e:放有肾脏的容器。f:去垢剂和缓冲液从肾动脉加入
Decellular ization methods of porcine kidneys for whole organ engineering using a high-throug hput system Biomaterials 33 (2012) 7756e7764 a:浓缩的去垢剂和缓冲液。b:去离子水加入。c:蠕动泵d:三通管 e:放有肾脏的容器。f:去垢剂和缓冲液从肾动脉加入
3D打印身体部位:从想象到现实 生物干细胞3D打印5年内进入动物实验阶段 牛细胞3D打印仿生耳可清晰收传声音 目前全球3D打印牙种植体的使用数量 3D打印仿生耳可听无线电频率 已经达到50万-75万个 三强图学出到
3D打印身体部位：从想象到现实 生物干细胞3D打印5年内进入动物实验阶段 牛细胞3D打印仿生耳可清晰收传声音 3D打印仿生耳 可听无线电频率 目前全球3D打印牙种植体的使用数量 已经达到50万-75万个
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues Nature Materials 11, 768-774(2012 Vascular unit cell Blood Interstitial zone Vascular wall CEGF interstitium HUVEC (mCherry Intervessel Vascular wall HUVEC jection Lumen ntervessel junction z-position= 1o um z-position= 30o um
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues Nature Materials 11, 768–774 (2012)