The eukaryotic transcriptional machinery contains polymerase and numerous proteins being organized to several complexes, such as the mediator and the tFInd complex Activators interact with one or more of these complexes and recruit them to the gene enhancer activator Mediator TFIID RNA polymerase Il TATA Figure 17-9
The eukaryotic transcriptional machinery contains polymerase and numerous proteins being organized to several complexes, such as the Mediator and the TFⅡD complex. Activators interact with one or more of these complexes and recruit them to the gene. Figure 17-9
Box 2 Chromatin Immuno-precipitation(ChIP) to visualize the recruitment (Where a given protein is bound in the genome of a living cell.) teins cross-linked antibody immunoprecipitate DNA-protein complex proteins removed amplify DNA by PCR ht o 2004 Pearson Education, Inc.. publishing as Benjamin cu
Box 2 Chromatin Immuno-precipitation (ChIP) to visualize the recruitment (Where a given protein is bound in the genome of a living cell.)
Activator Bypass Experiment-Activation of transcription through direct tethering of mediator to DNA.(很有应用价值 Directly fuse the Mediator bacterial DNA binding protein Gal11 LexA protein to the mediator complex Gall1 to LexA RNA polymerase ll activate GAL1 expression GAL 1 lexa site Copyright o 2004 Pearson Education, Inc, publishing as Benjamin Cummings Fiqure 17-10
Activator Bypass Experiment-Activation of transcription through direct tethering of mediator to DNA. (很有应用价值) Directly fuse the bacterial DNAbinding protein LexA protein to the mediator complex Gal11 to activate GAL1 expression. Figure 17-10
At most genes, the transcription machinery is not prebound, and appear at the promoter only /upon activation. Thus, /no allosteric activation of the prebound polymerase has been evident in eukaryotic requlation
At most genes, the transcription machinery is not prebound, and appear at the promoter only upon activation. Thus, no allosteric activation of the prebound polymerase has been evident in eukaryotic regulation
2-2 Activators also recruit modifiers that help the transcription machinery bind at the promoter 1. Modifiers direct recruitment of the transcriptional machinery 2. Modifiers help activate a gene inaccessibly packed within chromatin
2-2 Activators also recruit modifiers that help the transcription machinery bind at the promoter 1. Modifiers direct recruitment of the transcriptional machinery 2. Modifiers help activate a gene inaccessibly packed within chromatin