activating- reqion Gal4 Domain swap DNA-binding domain experiment Gal4 site Moving domains among proteins, proving that domains can be OFF dissected into Gal4 site separate parts of the proteins DNA-binding domai lacz Many similar OFF LexA site experiments shows Gal4 activating\ that dna binding reqion domains and LexA dNA binding domain activating regions LexA site are separable. 9 2004 Pearson Education, Inc, publishing as Benjamin
Domain swap experiment Moving domains among proteins, proving that domains can be dissected into separate parts of the proteins. Many similar experiments shows that DNA binding domains and activating regions are separable
Box I The two hybrid Assay is used to identify proteins interacting with each other. activating region gene NO transcription DNA-binding site DNA-binding domain gene NO transcription B gene transcription DNA-binding site Copyright o 2004 Pearson Education, Inc, publishing as Benjamin Cummings
Box 1 The two hybrid Assay is used to identify proteins interacting with each other
1-2 Eukaryotic regulators use a range of dNa binding domains, but DNA recognition involves the same principles same found in bacteria ● Homeodomain proteins e Zinc containing DNA-binding domain zinc finger and zinc cluster e Leucine zipper motif Helix-Loop-Helix proteins: basic zipper and HLh proteins
1-2 Eukaryotic regulators use a range of DNA binding domains, but DNA recognition involves the same principles same found in bacteria Homeodomain proteins Zinc containing DNA-binding domain: zinc finger and zinc cluster Leucine zipper motif Helix-Loop-Helix proteins : basic zipper and HLH proteins
Bactrial regulatory proteins Most use the helix-turn-helix motif to bind DNA target Most bind as dimers to DNA sequence each monomer inserts an a helix into the major groove Eukaryotic regulatory proteins 1. Recognize the DNA using the similar principles, with some variations in detail 2. Some form heterodimers to recognize DNA, extending the range of DNA binding specificity
Bactrial regulatory proteins • Most use the helix-turn-helix motif to bind DNA target • Most bind as dimers to DNA sequence: each monomer inserts an a helix into the major groove. Eukaryotic regulatory proteins 1. Recognize the DNA using the similar principles, with some variations in detail. 2. Some form heterodimers to recognize DNA, extending the range of DNAbinding specificity
Homeodomain proteins: The homeodomain is a class of helix-turn-helix DNA-binding domain and recognizes DNA in essentially the same way as those bacterial proteins Ser rg 2 As Figure 17-5 ucation, Inc, publishing as Benjamin Cummings
Homeodomain proteins: The homeodomain is a class of helix-turn-helix DNA-binding domain and recognizes DNA in essentially the same way as those bacterial proteins Figure 17-5