Agricultural Biotechnology crossbreeding Recombinant dna Species Related Related/unrelated Efficiency Susceptibility to external gene influences Genes shuffled in one 10sof1000s 1-few expriment Random insertion Insecti and herbicides Fertilizers Pollution/run off Potential toxins /risk 十 十 http://ww.insp.mx/xcongresolponencias/5
Agricultural Biotechnology Crossbreeding Recombinant DNA Species Related Related/unrelated Efficiency Susceptibility to external gene influences Genes shuffled in one exepriment 10s of 1000s 1 – few Random insertion + + Insecti and herbicides Fertilizers Pollution/run off Potential toxins/risk + + http://www.insp.mx/xcongreso/ponencias/5
Introducing genes into plants 1)infecting plant cells with plasmids as vectors carrying the desired gene Restriction Selectable enzyme site marker Restriction ORI enzyme site Restriction Restriction enzyme site enzyme site 2) shooting microscopic pellets containing the gene directly into the cell
Introducing genes into plants 1) infecting plant cells with plasmids as vectors carrying the desired gene; 2) shooting microscopic pellets containing the gene directly into the cell
Genetic engineering of plants with Agrobacterium tumefaciens A, tumefaciens, used extensively for genetic engineering of plants Contains T-DNA (bacterial plasmid) Genes colud be integrated into the plant chromosomes when the T-dna is Tumor induced by A. tumefaciens transferred http://courses.washingtonedu/z490/gmo/natural.html
Genetic engineering of plants with Agrobacterium tumefaciens • A. tumefaciens: used extensively for genetic engineering of plants. • Contains T-DNA (bacterial plasmid) • Genes colud be integrated into the plant chromosomes when the T-DNA is transferred. http://courses.washington.edu/z490/gmo/natural.html Tumor induced by A. tumefaciens
A Tumefaciens gall is not a tiny thing teide P profed E copyright: IFFF(BOKU)@ 1996 Reg dtu f califor
A.Tumefaciens gall is not a tiny thing
Biology of A tumefaciens Well known to induce crown gall tumor A tumefaciens lives around root surfaces(in rhizosphere) where it using nutriel that leak from the root tissues 谷 infects only through wound sites and actively chemotactic to them Www-genvagar.slu.se/teknik/ djup/plasm. htm Bacterial T-plasmid produces Plant wound produces receptors for acetosyringone COCH acetosyringone CH, O OCH
Biology of A. tumefaciens www-genvagar.slu.se/teknik/ djup/plasm.htm Well known to induce crown gall tumor A.tumefaciens lives around root surfaces (in rhizosphere) where it using nutrients that leak from the root tissues infects only through wound sites and actively chemotactic to them Plant wound produces acetosyringone Bacterial T-plasmid produces receptors for acetosyringone