(2)In vitro, (Polymerization) both ends of the mfgrow, but the plus end faster than the minus Because actin monomers tend to add to a filament 's plus end and leave from its minus end---r 0×080
(2) In vitro, (Polymerization) both ends of the MF grow, but the plus end faster than the minus. Because actin monomers tend to add to a filament ’ s plus end and leave from its minus end----
(3)Dynamic equilibrium between the g-actin and polymeric forms, which is regulated by atP hydrolysis and G-actin concentration ADPATP actin with actin with bound ADP bound ATP
(3) Dynamic equilibrium between the G-actin and polymeric forms, which is regulated by ATP hydrolysis and G-actin concentration
2.2 Assembly Mechanism of actin polymerization: 3 phases of G- actin polymerization Q Critical concentration(Cc). In steady state, G-actin monomers only exchange with subunits at the filament ends but there is no net change in the total mass of filaments During the elongation state, one end of the filament the (+)end elongates five to ten times faster than does the opposite ()end. This is because Cc value is much lower for G-actin addition at the(+)end than for addition at the(-)end
2.2 Assembly ◆ Mechanism of actin polymerization: 3 phases of Gactin polymerization. ◆ Critical concentration (Cc). In steady state, G-actin monomers only exchange with subunits at the filament ends but there is no net change in the total mass of filaments. ◆ During the elongation state, one end of the filament, the (+) end, elongates five to ten times faster than does the opposite (-) end. This is because Cc value is much lower for G-actin addition at the (+) end than for addition at the (-) end
Nucleus Nucleus Nucleus Factin G-actin @(lend (+1 endo Nucleation Elongation Steady state Elongation-外k- Steady state-→ Filament + nucle Monomer Actin concentration Figure 6-17 The three phases of G-actin polymerization in vitro
Figure 6-17 The three phases of G-actin polymerization in vitro
(4) Dynamic equilibrium is required for the cell functions. Some MFs are temporary and others permanent. (B) (C)
(4) Dynamic equilibrium is required for the cell functions. Some MFs are temporary and others permanent