Avery- Macleod- McCarty实验 rough strain smooth strain heat-klled rough strain 6 smooth strain heat-killed smooth strain mouse lives mouse dies mouse lives mouse dies
Avery–MacLeod–McCarty实验
遗传物质DNA Thymine Adenine DNA-Lgase DNA-Polymerase ( Pola 、护 DNA Polymerase ( Pol 1. Nucl 8. Smooth endoplasmic reticulum 2. Nucleus Cytosine 3. Ribosome 4. Vesicle 11.05 Guanine 5'end 5. Rough endoplasmic reticulum 12 Lysosome 6. Golgi apparatus Lennon 7. Cytoskeleton
遗传物质-DNA
噬菌体与细菌质粒 Cell replication ● myc epitope 6xHis 是是污 DNA pcDNATM3.1/ lc。ll Protein a myc-His/lacZ 8.5kb
噬菌体与细菌质粒
分子生物学重要的酶 限制性内切酶: Restriction enzymes→特异性切割DNA(ste- specific deoxyribonuclease) 连接酶 DNA ligase→非特异性连接二个DNA片段 多聚酶 DNA-directed DNA polymerase→以DNA为模板,合成新的DNA分子 逆转录酶: Reverse transcriptase→以RNA为模板,合成新的DNA分子 (RNA-dependent dNa polymerase) GAATTC CC CGGG CTTAAG GGGCCC
分子生物学重要的酶 • 限制性内切酶: Restriction enzymes →特异性切割DNA (site-specific deoxyribonuclease) • 连接酶:DNA ligase →非特异性连接二个DNA片段 • 多聚酶:DNA-directed DNA polymerase → 以DNA为模板，合成新的DNA分子 • 逆转录酶: Reverse transcriptase → 以RNA为模板，合成新的DNA分子 (RNA-dependent DNA polymerase)
Tag Polymerase CIENce hotoLDRARY Tom Brock discoverer of Tag I bacterium
Taq Polymerase Tom Brock, discoverer of Taq I bacterium