Relative volumes occupied by the major intracellular compartments in Liver Cell Intracellular compartment o of total cell volume Cytosol 54 Mitochondria 22 Rough er cisternae Smooth er cisternae plus Golgi cisternae 966 Nucleus Peroxisome lysosomes Endosomes
Intracellular compartment % of total cell volume Cytosol 54 Mittchondria 22 Rough ER cisternae 9 Smooth ER cisternae plus Golgi cisternae 6 Nucleus 6 Peroxisome 1 Lysosomes 1 Endosomes 1 Relative volumes occupied by the major intracellular compartments in Liver Cell
C. The Dynamic Nature of the Endomembrane system Most organelles are part of a dynamic system in which vesicles move between compartments o Biosynthetic pathways move proteins, carbohydrates and lipids within the cell Secretary pathways discharge proteins from cells Endocytic pathways move materials into cells Sorting signals are recognized by y receptors and target proteins to specific sites
C. The Dynamic Nature of the Endomembrane System vMost organelles are part of a dynamic system in which vesicles move between compartments. vBiosynthetic pathways move proteins, carbohydrates and lipids within the cell. vSecretary pathways discharge proteins from cells. vEndocytic pathways move materials into cells. vSorting signals are recognized by receptors and target proteins to specific sites
A few approaches to the study of cytomembranes Insights gained from autoradiography; Insights gained from the biochemical analysis of subcellular fractions Insights gained from the study of genetic mutants; The dynamic activities of endomembrane systems are highly conserved despite the structural diversity of different cell types
D. A few approaches to the study of cytomembranes v Insights gained from autoradiography; v Insights gained from the biochemical analysis of subcellular fractions; vInsights gained from the study of genetic mutants; The dynamic activities of endomembrane systems are highly conserved despite the structural diversity of different cell types
De duve. A Claude and G Palade. 1974 Nobel prize Golgi vesicles Secret Time of chase (min) Lumen Golgi comple Endoplasmic reticulum Mitochondna 20 min 120 min
De Duve, A.Claude and G.Palade,1974 Nobel Plrize
2. The structure and functions of Endoplasmic Reticulum(ER) inner nuclear membrane ER membrane nucleus outer nuclear membrane polyribosome RER has ba ribosomes on the cvtosolIc de of continuous flattened sacs (cisternae): A) 200nm 400nm SER IS an 5(B) rough ER smooth印R Interconne cting network of tubular membrane elements o0 nm ER lumen
2. The structure and functions of Endoplasmic Reticulum(ER)