《医学遗传学 Medical Genetics》课程PPT教学课件（英文版）Therapy of the Hereditary Disease

Gene Delivery Method Ex vivo Pu Controlled process; transfected cells are selected and expanded; more manipulations Pu Cells are usually autologous; they are then returned back to the patient

 Controlled process; transfected cells are selected and expanded; more manipulations  Cells are usually autologous; they are then returned back to the patient Gene Delivery Method ❖ Ex vivo

Physical Methods Direct injections XX 冷 Electroporation %s Microparticle bombardment (g ene gu

Physical Methods ❖ Direct injections ❖ Electroporation ❖ Microparticle bombardment (gene gun)

Chemical methods Coprecipitation with calcium phosphate Pu Transfer efficiency: 1%00. 1%0

Chemical Methods ❖ Coprecipitation with calcium phosphate  Transfer efficiency: 1% ~0.1%

Membrane fusion Artificialliposomes ☆ Erythrocyte ghosts Microcell Spheroplast

Membrane Fusion ❖ Artificial liposomes ❖ Erythrocyte ghosts ❖ Microcell ❖ Spheroplast