Chapter g Electron transport and oxidative phosphorylation
Chapter 9 Electron transport and oxidative phosphorylation
第一节Ou| ine of bio- oxidation 切生命活动都需要能量。 所有生物都可以看成是能量的转换者。 这种能量的流动驱动着生命的维持与繁衍
一切生命活动都需要能量。 所有生物都可以看成是能量的转换者。 这种能量的流动驱动着生命的维持与繁衍。 第一节 Outline of Bio-oxidation
Some historical facts about our understanding on oxidative phosphorylation 1930s: Pyruvate was known to be completely oxidized to co2 via the citric acid cycle(with O2 consumed) 1930s: NAD+ and Fad were found to be e carriers between metabolites and the respiratory chain 1930s: Role of atp and general importance of phosphorylation in bioenergetics were realized
Some historical facts about our understanding on oxidative phosphorylation • 1930s: Pyruvate was known to be completely oxidized to CO2 via the citric acid cycle (with O2 consumed). • 1930s: NAD+ and FAD were found to be ecarriers between metabolites and the respiratory chain. • 1930s: Role of ATP and general importance of phosphorylation in bioenergetics were realized
1950s: Isolated mitochondria were found to effect the obligatory coupling of the phosphorylation of adp and the e- transfer from NADH to O2 1961, the chemiosmotic hypothesis was proposed for linking the e- transfer and ADP phosphorylation(based on the uncoupling phenomenon and the intactness requirement) 1960s, ATP synthase was identified from mitochondria
• 1950s: Isolated mitochondria were found to effect the obligatory coupling of the phosphorylation of ADP and the e- transfer from NADH to O2 . • 1961, the chemiosmotic hypothesis was proposed for linking the e- transfer and ADP phosphorylation (based on the uncoupling phenomenon and the intactness requirement) • 1960s, ATP synthase was identified from mitochondria
The Overall picture of oxidative phosphory lation.It occurs on the of Intermembrane mitochondria or the plasma space membrane of bacteria) mitochondna 1930s Transporter Citric acid cy (glycolysis) OO HSC。A P+ GDP GTP 1930s Pyruvate CHa-C-C-OH SCOA 2C2 Acetyl CoA 3NAD3NADH FAD ATP+ AM HSCOA →FADH2 HSCOA/ PP 7 Fatty acyl atty acyl FAD Mitochondrial Transporter NADH Shuttle NADH NAD+ 1940s-1950s ATP ADP ADP complex FADH2 P 2e+H H2o NADH NAD NAD FAD H 1960s Oxidative phosphorylation Electron Electron transport via the transport complexes respiratory chain Transport of metabolites Pyruvate dehydrogenase and citric acid cycle into the mitochondrion ATP synthesis by FoF Fatty acid metabolism using proton-motive force Electron transport from NADH and FADH. to ATP export, ADP and P, import oxygen; generation of proton-motive force
The Overall picture of oxidative phosphorylation It occurs on the cristae of mitochondria or the plasma membrane of bacteria) 1930s 1960s 1930s (glycolysis) 1940s-1950s Electron transport via the respiratory chain Oxidative phosphorylation