、丙酮酸氧化脱羧 Pyruvate loses COa and e Hydroxyethyl group is Acetyl group is transferred HETPP is formed transferred to lipoic acid to CoA and oxidized to form acetyl dihydrolipoamide CoASH CH.C-SCOA 4 Dihydrolipoamide CHe-c is reoxidized CH -C-COo Thiamine Pyruvate SH NADY pyRophosphate SH IFAD Protein Hs NADH H CH-OH TPP HvdiroxvethyI TPP Lipoic acicl (HETPP) urinate Dihydrolipoyl Dihydrolipoyl tlehvelrogenas Irnsacetslas clelvdraenase
一、丙酮酸氧化脱羧
Pyruvate dehydrogenase complexes from E coli the electron micrograph 来部 a huge multimeric assembly of three kinds of enzymes having 60 subunits in bacterial and more in mammals (a) 0.05m
Pyruvate dehydrogenase complexes from E. coli: the electron micrograph a huge multimeric assembly of three kinds of enzymes, having 60 subunits in bacteria and more in mammals
ruvate co, Acetyl-CoA E2(dihydrolipoyl transacetylase) consisting the core, 24 subunits HydroxyethVl-TPP E(pyruvate dehydrogenase) bound to the e2 core, 24 subunits E 3 E3 (dihydrolipoyl dehydrogenase) bound to the e core. 12 subunits (b) A model of the E coli pyruvate dehydrogenase complex showing the three kinds of enzymes and the flexible lipoamide arms covalently attached to e
A model of the E. coli pyruvate dehydrognase complex showing the three kinds of enzymes and the flexible lipoamide arms covalently attached to E2 E2 (dihydrolipoyl transacetylase): consisting the core, 24 subunits; E1 (pyruvate dehydrogenase): bound to the E2 core, 24 subunits; E3 (dihydrolipoyl dehydrogenase): bound to the E2 core, 12 subunits. Pyruvate E2 E3 Hydroxyethyl-TPP CO2 Acetyl-CoA
Reactive thiol group NH2 H H CH3 aDenine 5 HS-CH2-CH2-N-C-CH2-CH2-N-C-C-C-CH2-0-P-0-P-0-CH20 N-N B-Mercapto- O OHCH3 ethylamine H Pantothenic acid Ribose-3-phosphate 0 OH O=P-0 CHa-C O S-COA 3.Phosphoadenosine diphosphate AcetylCoA C zvme Coenzyme A (CoA-sH): discovered in 1945 by Lipmann, delivers activated acyl groups(with 2 carbons) for degradation or biosynthesis
Coenzyme A (CoA-SH): discovered in 1945 by Lipmann, delivers activated acyl groups (with 2-24 carbons) for degradation or biosynthesis
1.化学反应过程 TCA环 三羧酸循环( tricarboxylic acid cycle),又叫做 TCA循环,是由于该循环的第一个产物是柠檬酸,它 含有三个羧基,故此得名。 该循环的提出的主要贡献者是英国生化学家 Krebs,所以叉称 Krebs循环。 该循环还叫做柠檬酸循环
三羧酸循环(tricarboxylic acid cycle)，又叫做 TCA循环，是由于该循环的第一个产物是柠檬酸，它 含有三个羧基，故此得名。 该 循 环 的提 出 的 主要 贡 献者 是 英 国生 化 学家 Krebs，所以又称Krebs循环。 该循环还叫做柠檬酸循环。 1. 化学反应过程 二、TCA循环