PCR反应体系的五要素 引物、酶、dNTP、模板和Mg2+ Tag dNA聚合酶 来自水生栖热菌7 hermusaguaticus 良好的耐热性 Mg2+依赖性 无校读功能
PCR反应体系的五要素： 引物、酶、dNTP、模板和Mg2+ Taq DNA聚合酶 来自水生栖热菌 Thermusaquaticus 良好的耐热性 Mg2+依赖性 无校读功能
PCR反应体系的准备 +Taq polymerase Thermus aquaticus +nucleotides t primers salts(ions) dna fragment
PCR反应体系的准备 + primers + nucleotides +Taq polymerase (Thermus aquaticus ) + salts (ions) + DNA fragment
Step 1: Denaturation变性 94°C TGAACGTAGTAGCATAGCAATGTACATCATCAGATAGAC GATTGACAGTGATCTCGACGATGCATCATGACATCGATTTGATCGAT ACTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGCTAAACTAGCTA
TGAACGTAGTAGCATAGCAATGTACATCATCAGATAGACGATTGACAGTGATCTCGACGATGCATCATGACATCGATTTGATCGAT ACTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGCTAAACTAGCTA Step 1 : Denaturation 变性 94°C
Step2: Annealing退火 primer-dependent TGAACGTAGTAGCATAGCAATGTACATCATCAGATAGAC GATTGACAGTGATCTCGACGATGCATCATGACATCGATTTGATCGAT GCTGCTACGTAG CGTAGTAGCA CTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGC TAAACTAGCTA
TGAACGTAGTAGCATAGCAATGTACATCATCAGATAGACGATTGACAGTGATCTCGACGATGCATCATGACATCGATTTGATCGAT ACTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGCTAAACTAGCTA Step 2 : Annealing 退火 T° primer-dependent CGTAGTAGCA GCTGCTACGTAG
Step3: Extension延伸 72°C Taq polyme IGAACGCAGCAGCAHAGCAATACACAG2:28平82C TACGATGCATCATGACATCGATTTGATCGAT GCTGCTACGTAG CGTAGTAGCA TAGCAATGTACATCATCAGATAGACGATTGACAGTGATCTCGACGATGCATC CTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGC TAAACTAGCTA Taq polymera
TGAACGTAGTAGCATAGCAATGTACATCATCAGATAGACGATTGACAGTGATCTCGACGATGCATCATGACATCGATTTGATCGAT ACTTGCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGAGCTGCTACGTAGTACTGTAGCTAAACTAGCTA Step3 : Extension 延伸 72°C CGTAGTAGCA GCTGCTACGTAG Taq Polymerase Taq Polymerase TAGCAATGTACATCATCAGATAGACGATTGACAGTGATCTCGACGATGCATC GCATCATCGTATCGTTACATGTAGTAGTCTATCTGCTAACTGTCACTAGA