RNA Poly(A>t TTTAAAAA viral reverse franscnptase Oligo(dT primer RNA 八A八八点AAA CDNA Hairpin loop NaOH TTTT DNA polymerase I CDNA S1 nuclease singie-strand-specitic) Double-stranded CDNA
Nucleic acid Probe A labelled segment of DNA (RNA) that is able, after a dNa hy bridization reaction to detect a specific Dna sequence in a mixture of sequences
Nucleic Acid Probe A labelled segment of DNA (RNA) that is able, after a DNA hybridization reaction, to detect a specific DNA sequence in a mixture of sequences
Type of Probe DNA probe ☆ RNA probe s Oligonucleotide probe Pu Degenerate probe Pu Unique EST-base probe
Type of Probe ❖ DNA probe ❖ RNA probe ❖ Oligonucleotide probe Degenerate probe Unique EST-base probe
Degenerate Probe Leuˇcysy、 tYr MetHis Gl Asps er CTA TGC AT C TA C ATG CAC CAG GAC TCA TT IAG C
Degenerate Probe Leu Cys Ile Tyr Met His Gln Asp Ser CT T C A G TGT C AT T C A TA T C CAT ATG C CAA G GAT C TC T C A G TT A G AGT C
Type of Probe DNA probe ☆ RNA probe s Oligonucleotide probe Pu Degenerate probe Pu Unique EST-base probe
Type of Probe ❖ DNA probe ❖ RNA probe ❖ Oligonucleotide probe Degenerate probe Unique EST-base probe