Moist hea湿热: denature proteins and melt lipids more effective □ Autoclaving:121°C,1034kPa,20min cidal for both vegetative organisms and endospores 口 Boiling water煮沸灭菌 口 Pasteurization巴氏消毒法: to kill particular spoilage organisms or pathogens ■ flash method瞬间法:71.6°C,15s ■ holding method持续法:62.9°C,30min
◼ Moist heat湿热：denature proteins and melt lipids; more effective Autoclaving: 121℃,103.4kPa, 20min cidal for both vegetative organisms and endospores Boiling water煮沸灭菌 Pasteurization巴氏消毒法: to kill particular spoilage organisms or pathogens ◼ flash method瞬间法: 71.6°C, 15s ◼ holding method持续法: 62.9°C, 30 min
fractional sterilization间歇蒸气灭菌法 1) Steam heating to100°cfor30min—常压/流动蒸气消毒法 vegetative cells are destroyed but endospores survive 2) Incubate at30°c37° C overnight Most bacterial endospores germinate 3 Second heat treatment, 100 C, 30 min Germinated endospores are killed 4) Second incubation at30°c-37° C overnight Remaining endospores germinate 5)Third heat treatment, 100 c, 60 min Last remaining germinated endospores are killed
fractional sterilization间歇蒸气灭菌法 : 1) Steam heating to 100 °C for 30 min——常压/流动蒸气消毒法 Vegetative cells are destroyed but endospores survive 2) Incubate at 30 °C -37 °C overnight Most bacterial endospores germinate 3) Second heat treatment, 100 °C, 30 min Germinated endospores are killed. 4) Second incubation at 30°C-37 °C overnight Remaining endospores germinate 5) Third heat treatment, 100 °C, 60 min Last remaining germinated endospores are killed