一、种间杂交与生殖隔离 不可交配性（non-crossability) 育种家用这一术语来判断种间杂交 时能否获得有生命力的杂交种子的情况， 不深究是受精前障碍还是受精后障碍在 起作用。 Biology of plant reproduction
Biology of plant reproduction 11 一、 种间杂交与生殖隔离 ▲ 不可交配性（non-crossability） 育种家用这一术语来判断种间杂交 时能否获得有生命力的杂交种子的情况， 不深究是受精前障碍还是受精后障碍在 起作用
二、种间杂交不亲和性 (interspecific incompatibility) 1. 定义definitions 两个可育(fertile)的不同种植物受粉 后，花粉不能萌发，或花粉管行为反常， 或雌雄间不相容而导致杂种合子不能形 成的现象。 Biology of plant reproduction WW
Biology of plant reproduction 12 二、种间杂交不亲和性（interspecific incompatibility） 1. 定义definitions 两个可育(fertile)的不同种植物受粉 后，花粉不能萌发，或花粉管行为反常， 或雌雄间不相容而导致杂种合子不能形 成的现象
二、种间杂交不亲和性interspecific incompatibility 2.表达部位position ●柱头不亲和(stigma incompatibility):花粉不 萌发或萌发后不伸长。主要发生在三核花粉和 干柱头(solid type)的科属 ●花柱不亲和(style incompatibility):花粉可萌 发但在花柱中伸长受阻。主要发生在二核花粉 和湿柱头((the open type))的科属中。 Biology of plant reproduction
Biology of plant reproduction 13 二、种间杂交不亲和性interspecific incompatibility） 2. 表达部位position ●柱头不亲和(stigma incompatibility)：花粉不 萌发或萌发后不伸长。主要发生在三核花粉和 干柱头(solid type)的科属 ●花柱不亲和(style incompatibility)：花粉可萌 发但在花柱中伸长受阻。主要发生在二核花粉 和湿柱头(the open type)的科属中
二、种间杂交不亲和性interspecific incompatibility 胚囊不亲和(embryo sac incompatibility): ●) 花粉管伸进胚囊，并释放出精子，但精 子不能有效地和雌配子结合实现双受精， 或者是仅发生精子与极核融合的单受精。 Biology of plant reproduction
Biology of plant reproduction 14 二、种间杂交不亲和性interspecific incompatibility） ●胚囊不亲和(embryo sac incompatibility)： 花粉管伸进胚囊，并释放出精子，但精 子不能有效地和雌配子结合实现双受精， 或者是仅发生精子与极核融合的单受精
Chinese Science Bulletin ©2007 色 Science in China Press 2 Springer-Verlag Cytological mechanisms of interspecific incrossability and hybrid sterility between Oryza sativa L.and O. alta Swallen FU XueLin,LU YongGen'.LIU XiangDong,Li JinQuan Feng JiuHuan Guangdong Provincial Key Laboratory of Plant Molecular Breeding,South China Agriculture University,Guangzhou 510642.China Oryza sativa and O.alta belong to AA and CCDD genomes in Oryza,respectively.Interspecific repro- ductive isolation limits the transfer of favorable genes from O.alta into O.sativa.The cytological mechanisms of interspecific incrossability and hybrid sterility between O.sativa and O.a/ta were studied systematically in this paper.We indentified two cytological causes of interspecific incrossabil- ity.First,we observed embryo sac incompatibility that caused fertilization barriers of variable severity such as non-fertilization,fertilization stagnation and egg cell single-fertilization.Second,we observed hybrid inviability,the major cause for incrossability,apparent from hybrid embryo developmental stagnation and embryo abortion.Hybrid sterility included both embryo sac sterility and pollen sterility. The hybrid embryo sac was completely sterile and exhibited mainly embryo sac degeneration.Hybrid pollen was also sterile and mainly typical abortive.Hybrid sterility was mainly caused by severely ab- normal meioses of megasporocytes and pollen mother cells;it is the most important abnormality,being chromosome sterility.Several methods are suggested to overcome the interspecific reproductive iso- lation between O.sativa and O.alta
Biology of plant reproduction 15