3)capacitation of spermatozoa o decapacitation factor: exist in epididymus, to inhibit the release of acrosome enzvmes e capacitation factor: secreted by epi cells of oviduct, capacity of releasing acrosome enzvmes
3) capacitation of spermatozoa: decapacitation factor: exist in epididymus, to inhibit the release of acrosome enzymes capacitation factor: secreted by epi. cells of oviduct, capacity of releasing acrosome enzymes
4)quality and quantity of spermatozoa 2-6ml,100.000.000/ml <1.5ml;or<10,000,000; abnorma sperm >30%,or/capacity for mobile< 70 5)meeting of sperm and ovum ● sperm:20-24h ●ovum:15-18h(1-3ds)
4) quality and quantity of spermatozoa: 2-6 ml, 100,000,000/ml, <1.5 ml; or <10,000,000; abnormal sperm >30%; or capacity for mobile< 70% 5) meeting of sperm and ovum sperm: 20-24h ovum: 15-18h(1-3ds)
获能后 透明带 processes of fertilization 一穿入后的精子尾 acrosome reaction 子头部 /hyaluronic acidase: dissolve corona radiate interact with ZP3(receptor glycoprotein, exist in zona pellucid) molecules, release acrosin dissolve zona pellucid enter perivitelline space
---processes of fertilization: 1) acrosome reaction: /hyaluronic acidase: dissolve corona radiate * interact with ZP3(receptor glycoprotein, exist in zona pellucide) molecules, release /acrosin: dissolve zona pellucide /enter perivitelline space
2)spermatozoa fuse with the membrane of ovum: nucleus enter cytoplasm of ovum 3)monospermy cortical reaction: cortical granules(located in cytoplasm of ovum)/are released into perivitelline space, and cause zona reaction: change of ZP3 molecules in zona pellucid 4) formation of zygote(fertilized ovum secondary meiosis complete: form second polar body male pronucleus fuse with female pronucleus, to restore the 2n chromasome
2) spermatozoa fuse with the membrane of ovum: nucleus enter cytoplasm of ovum 3) monospermy: cortical reaction: cortical granules(located in cytoplasm of ovum) are released into perivitelline space, and cause zona reaction: change of ZP3 molecules in zona pellucide 4) formation of zygote(fertilized ovum) secondary meiosis complete: form second polar body male pronucleus fuse with female pronucleus, to restore the 2n chromasome
significance of fertilization 1)a new life begin (initiation of cleavage) 2)restoration of the diploid number of chromosomes: cross-over 3)determination of the sex of the new individual e ova (22+X)+ X-bearing spermatozoon girl e ova (22+X)+ Y-bearing spermatozoon boy *klinefelter's syndrome: 47XXY, 48XXXY
---significance of fertilization: 1) a new life begin(initiation of cleavage) 2)restoration of the diploid number of chromosomes: cross-over 3) determination of the sex of the new individual: ova(22+X) + X-bearing spermatozoon = girl ova(22+X) + Y-bearing spermatozoon = boy *klinefelter’s syndrome: 47XXY, 48XXXY