注意：(1)最初的培养基必须是非选择性的，即各种突变型都能够在其上生长；(2)必须采用适当的方法如涂布或划线法，以使培养物菌落之间要分开。16
16 (2)必须采用适当的方法如涂布或划线法，以使培养物菌 落之间要分开。 注意： (1)最初的培养基必须是非选择性的，即各种突变型都能 够在其上生长；
影印培养法sterilovolvetmastorplate wichbacterialcoloriesgrownonnonseiectivemediun菌落aAsterilepiecedfvehvetispressedonamasterplatewithtacterialcoloniesgrowngon.nonselectivemedium.Tnevetvetpicksupceflsfromthocolones母板--teplica plate withselectivomodiumb The volvet ispressodontoateplicapliatewith.selectivemediurn so tnat itplaces celis on thepiate inthe samepositionsasthecolonios onthemaster plateCells trom mutantMutantcolorycolonycanbefals.to.growoncollectedfiomthie印影琼脂板roplicaplate.masterplate图7-3细菌的影印培养teplicaplatemastetplatecTrece'son.thereplicaplatearealtiowedtogrowintocoionies.Anycolonypresentonthemasterplatebutmissingtromthereplicaplatehasamutationthatprevents itsgrowthontheSclectivemedium.Cesfrom.tremutantcolonycanthenbecotected trom themasterplate
17 影印培养法