Read Kuby pages 109-110:Multigene Organization of Ig Genes (a)A-chain DNA LV22 Jx2 Cx2 J4 C24 LVx1 Jx3 Cx3 J1 C.1 5☐ 701.2 2.01.3 191.41.713 kbkb kbkb kbkb kbkb (b)K-chain DNA n=-85 LV1 LV 2 LVen (4 ☐☐… 1-181☐3 23 2.5 kb kb (c)Heavy-chain DNA n=-134 LVH1 LVHn DH1 DH13 JH1 JH4 Cu C8 C3 C,1 C 2b Cy2a Ce ☐…a∥-…H☐ -3 6.54.5 55 34 21 15 14 12 kbkb kb kb kb kbkb kb Kuby Figure 5-3
Read Kuby pages 109-110: Multigene Organization of Ig Genes Kuby Figure 5-3
(b) Antigen binding Biological activity CHO CHO 6
VDJ--> W- C-- c-->C In heavy chains,the V,D and J segments encode the variable domain while the C segment encodes the constant domain. In light chains,the V and J segments encode the variable domain while the C segment encodes the constant domain
In heavy chains, the V, D and J segments encode the variable domain while the C segment encodes the constant domain. In light chains, the V and J segments encode the variable domain while the C segment encodes the constant domain
During B cell maturation,immunoglobulin genes undergo rearrangement. 1.D-->JH Rearrangement H D JH Cy3 Cy1 Cy2b Cy2a Ca 5'32☒什H州■■■ -3 A section containing a number of D regions and a number of Jregions loops out H Cs 5'☒2☒☐中H册
During B cell maturation, immunoglobulin genes undergo rearrangement
During B cell maturation,immunoglobulin genes undergo rearrangement. 1.D-->JH Rearrangement D JH Cμ Cs Cy3 Cy1 Cy2b Cy2a 5'32☒什州■■■ A section containing a number of D regions and a number of J regions loops out Cμ Cμ 5'3☒2☒□HH州 5'☒2☒HHH Excision of the loop Result:Apposition of a D and J region
During B cell maturation, immunoglobulin genes undergo rearrangement