2. BLASt 序列相似性检索 Basic local alignment search Tool ■是核酸和蛋白质序列的局部对准相似 性检索工具 20219/19 复旦大学图书馆文献检索教研室
2021/9/19 复旦大学图书馆文献检索教研室 2. BLAST 序列相似性检索 ◼ Basic Local Alignment Search Tool ◼ 是核酸和蛋白质序列的局部对准相似 性检索工具
a NCBI Resources How To National Center for Biotechnology Information rch All Databases Resources Help Us Improve CBIHon Genome Reference Consortium All Resources (A-z Formed to Improve human and mouse reference Send us feedback on the new home Literature page and guide pages design DNA RNA alternate representations of complex loci Protein 12 NLMUNCBI H1N1 Flu Resources Genes Expression FLU.GOV HOw TO Know what to do Maps Markers obtain the full text of an article Domains structures Retrieve all sequences for an organism or taxon VsITFLUGOY Find a homolog for a gene in another organism Taxonomy Find genes associated with a phenotype or disease Newest H1N1 influenza sequence Data Sofware Design PCR primers and check them for specificity Submit flu sequences to Training Tutorials Find the function of a gene or gene product Find syntenic regions between the genomes of two organis Homology Latest H1N1 citations Small Molecules see (consumer health variatio Intormation About the NcBl Enviro-Health links he National Center for Biotechnology Information advances science and Popular Resources health by providing access to biomedical and genomic inform More about the NCBI I Mission I Organization I Research I RSS PubMed Central Nucleotide Conserved Domains Structure
序列相似性检索 ■ Blast是为从相同和不同的有机体中,提供 对比核酸或蛋白质序列,寻找相似性序列片 断的工具。 通过寻找不同基因的相同序列片段,可以推 断最新测定的基因功能、预测基因家族的新 成员、探索基因的进化关系,预测蛋白质代 码和翻译产物的功能和定位 20219/19 复旦大学图书馆文献检索教研室
2021/9/19 复旦大学图书馆文献检索教研室 序列相似性检索 ◼ Blast是为从相同和不同的有机体中，提供 对比核酸或蛋白质序列，寻找相似性序列片 断的工具。 ◼ 通过寻找不同基因的相同序列片段，可以推 断最新测定的基因功能、预测基因家族的新 成员、探索基因的进化关系，预测蛋白质代 码和翻译产物的功能和定位
习 BLAST [Sign Inl Register Home Recent Results Saved Strategies Help Basic Local Alignment Search Tool )NCBU BLAST Home 基因组对比 BLAST finds regions of similarity between biological sequences. mores ReWBLAST Redesign in Learn more about how to use the new BLAST design Old blast After beta testing the new BLAST AsSembled Genomes BLAST pages will become the efault BLAST portal a 6/2007 Choose a species genome to search, or list all genomic BLAST databases D Human D Oryza sativa a Gallus gallus a Mouse d Bos taurus a Pan troglodytes 0305/2007 Special Announcement: Beta Test of Rat Danio rerio a Microbes n Arabidopsis thaliana Drosophila melanogaster Apis mellifera 基本对比 test for a Basic BLAST 选择对比程序 Choose a BLAST program to rur nucleotide blast Search a nucleotide database using a nucleotide query A/gothams: blastn gablast, discontiguous megablast Tip of the Day protein blast Search protein database using a protein query How to save custom search blastx Search protein database using a translated nucleotide query thlastn Search translated nucleotide database using a protein query arches and after adjusting the tabase, organism limits and maybe a few Algorithm tblastx Search translated nucleotide database-trsing-atranstatetHmtreleotide 特殊对比 Parameters you arrive at what you think is a good search ategy. Do you want to have to Specialized BLAST dd e with pull down menus or emember all the changes you Choose a type of specialized search(or database name in parentheses. de the next time to want to o Search trace archives un a similar search? Now you
2021/9/19 复旦大学图书馆文献检索教研室 基本对比 选择对比程序 基因组对比 特殊对比
④剑⑨昌③画·目甾 弓 BLAST My NCBI Home Recent Results Saved Strategies Help Basic Local Alignment Search Tool b NCBU BLAST blastn suite: BLASTN programs search nucleotide databases using a nucleotide query. more. Enter Query Sequence Reset page Bookmark Enter accession number, gi, or FASTA sequence g Query subrange@ 241 ogctaccctg agtctgctgg cggggcaaac tgtagagctt agatgtaaag 9 301 tgggtogage taccetgegt atctggacac ctttaaggat tetegectea g 361 gaatgagcge tacggccagt toactctggt caactecace tomgcagaca 421 cagctgetgg gtgcagetct gcagoogcta catetgcagg aaqgacgagg 40] ctecacctac atetttttta cagagaaagg agaactettt gtaccttete ccagctactt 54: cgatqttqte tacttgaace oggacagaca ggctgtagtt ccttqtegg Or, upload file 浏览 Tit Enter a descriptive title for your BLAST search e Choose Search S Database C Human genomic +transcript Mouse genomic transcript c Others(nr etc. 将序列数据库中的复 Nucleotide collection(nr/nt 制序列在此粘贴 C Any C Human C A thaliana C Mouse ( Custom Search only sequences from selected organism 自动启动系统自动将 序列号贴在次框中 Entrez Query Enter an Entrez query to limit search e Program Selection ghly similar sequences(megabla 20219/19 复旦大学图书馆文献检索教研室
2021/9/19 复旦大学图书馆文献检索教研室 将序列数据库中的复 制序列在此粘贴 自动启动系统自动将 序列号贴在次框中